| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Peretti, Victoria I (8)
|
MATWAC |
39.40 |
39.66 |
0.26 |
| 2 |
Rodriguez, Neomi (8)
|
MANSC |
|
42.66 |
- |
| 3 |
Crouthamel, Lauren C (8)
|
MAWWC |
48.68 |
42.82 |
-5.86 |
| 4 |
Hutton, Ava L (8)
|
MAWAC |
47.34 |
43.44 |
-3.90 |
| 5 |
Kiesel, Addison E (8)
|
MASJAC |
47.23 |
43.83 |
-3.40 |
| 6 |
McKendrick, Ainsley K (8)
|
MATWAC |
|
45.76 |
- |
| 7 |
Geberth, Rebecca L (8)
|
MATHY |
51.83 |
46.55 |
-5.28 |
| 8 |
Moore, Pamela H (8)
|
MAWAC |
50.82 |
47.68 |
-3.14 |
| 9 |
Lotta, Grace K (8)
|
MATHY |
1:03.85 |
49.08 |
-14.77 |
| 10 |
Rice, Addison (8)
|
MAWAC |
51.70 |
49.90 |
-1.80 |
| 11 |
Kouser, Madeline E (7)
|
MASJAC |
|
50.31 |
- |
| 12 |
Colon, Karrington R (8)
|
MAGPAC |
59.96 |
50.67 |
-9.29 |
| 13 |
Landers, Meghan E (8)
|
MATHY |
48.04 |
51.01 |
2.97 |
| 14 |
Ventresca, Khloe R (8)
|
MAWAC |
54.97 |
52.22 |
-2.75 |
| 15 |
Carroll, Teagan R (8)
|
MASTRM |
53.61 |
52.57 |
-1.04 |
| 16 |
Devlin, Keira E (6)
|
MAWAC |
|
54.66 |
- |
| 17 |
DeVito, Isabella C (8)
|
MAGPAC |
55.08 |
56.17 |
1.09 |
| 18 |
Curtis, Mia S (8)
|
MAWAC |
|
57.27 |
- |
| 19 |
Morrow, Kylie E (7)
|
MAWAC |
1:05.77 |
58.59 |
-7.18 |
| 20 |
Hauske, Katelyn M (8)
|
MAWAC |
1:04.86 |
58.68 |
-6.18 |
| 21 |
Briscoe, Chloe R (6)
|
MAGPAC |
|
59.03 |
- |
| 22 |
Varani, Annalise R (8)
|
MATHY |
|
59.53 |
- |
| 23 |
Nunez-Ledesma, Amelie (8)
|
MANSC |
1:02.14 |
1:00.64 |
-1.50 |
| 24 |
Longo, Natalie G (7)
|
MASJAC |
|
1:02.32 |
- |
| 25 |
Joseph, Mia (7)
|
MASJAC |
|
1:03.16 |
- |
| 26 |
Liolios, Athena (8)
|
MASJAC |
|
1:03.40 |
- |
| 27 |
Behr, Samantha M (8)
|
MASJAC |
|
1:04.01 |
- |
| 28 |
Overton, Madison (8)
|
MASJAC |
|
1:05.43 |
- |
| 29 |
Bieg, Theresa B (7)
|
MASJAC |
|
1:06.46 |
- |
| 30 |
Kasthuri, Athira D (8)
|
MASJAC |
|
1:09.26 |
- |
| 31 |
Mitchell, Maura (7)
|
MASJAC |
|
1:14.01 |
- |
|
Gasparovic, Anastasia M (8)
|
MATWAC |
|
NS |
- |