| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rogers, Zoey S (10)
|
MAWAC |
2:38.90 |
2:40.68 |
1.78 |
| 2 |
Tran, Anna (10)
|
MAPVA |
2:50.07 |
2:48.10 |
-1.97 |
| 3 |
DeCardona, Sophia E (10)
|
MAGAAC |
3:00.45 |
2:49.31 |
-11.14 |
| 4 |
Seidman, Jordan K (10)
|
MAGAAC |
3:06.61 |
2:52.78 |
-13.83 |
| 5 |
Johnson, Eliza C (10)
|
MAWAC |
2:56.14 |
2:52.90 |
-3.24 |
| 6 |
Fohring, Keira E (10)
|
MASJAC |
|
2:54.21 |
- |
| 7 |
Aiello, Cameron J (10)
|
MAWAC |
3:03.68 |
2:54.36 |
-9.32 |
| 8 |
Baran, Georgia C (10)
|
MAGAAC |
|
2:54.42 |
- |
| 9 |
Ryan, Maxalyn E (9)
|
MAWAC |
3:04.78 |
2:59.83 |
-4.95 |
| 10 |
Radziewicz, Gabriella E (10)
|
MAWAC |
3:07.14 |
3:00.59 |
-6.55 |
| 11 |
Jefferson, McKinley F (9)
|
MAWAC |
3:16.46 |
3:01.15 |
-15.31 |
| 12 |
Ashman, Aubrey V (9)
|
MAWAC |
3:28.54 |
3:19.10 |
-9.44 |
| 13 |
Baughman, Caroline E (9)
|
MAGAAC |
3:28.22 |
3:26.45 |
-1.77 |