| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Devlin, Keira E (6)
|
MAWAC |
20.18 |
19.45 |
-0.73 |
| 2 |
Shur, Brenna R (6)
|
MASEA |
24.37 |
20.80 |
-3.57 |
| 3 |
Wainwright, Ella G (6)
|
MAECCC |
24.59 |
21.37 |
-3.22 |
| 4 |
Elberson, Mallory R (6)
|
MAECCC |
26.23 |
22.56 |
-3.67 |
| 5 |
Hutton, Ellie R (6)
|
MAWAC |
24.43 |
22.63 |
-1.80 |
| 6 |
Smith, Tristyn S (5)
|
MAGPAC |
26.20 |
25.79 |
-0.41 |
| 7 |
Resnick, Reese B (6)
|
MAECCC |
27.32 |
26.58 |
-0.74 |
| 8 |
Emmett, Reese A (6)
|
MAGPAC |
26.26 |
27.24 |
0.98 |
| 9 |
Nordberg, Alaina R (5)
|
MAWWC |
31.47 |
27.28 |
-4.19 |
| 10 |
Hauske, Megan L (5)
|
MAWAC |
30.90 |
27.59 |
-3.31 |
| 11 |
DeRogatis, Leah E (6)
|
MAPRSC |
50.00 |
27.65 |
-22.35 |
| 12 |
Bluemle, Greta J (6)
|
MAWAC |
35.85 |
28.31 |
-7.54 |
| 13 |
Hellmann, Anneliese M (6)
|
MAPRSC |
28.61 |
31.28 |
2.67 |
| 14 |
Boyer, Emma L (6)
|
MAWAC |
30.96 |
31.60 |
0.64 |
| 15 |
Grant, Jayden C (6)
|
MAWAC |
30.05 |
36.01 |
5.96 |
| 16 |
Gateretse, Isabelle L (4)
|
MAWAC |
41.14 |
40.76 |
-0.38 |