| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Somogyi, Addison N (10)
|
MDACA |
45.85 |
40.37 |
-5.48 |
| 2 |
Birdsall, Claire G (10)
|
MDEST |
43.90 |
46.56 |
2.66 |
| 3 |
Cai, Catherine N (10)
|
MDMAC |
45.16 |
46.88 |
1.72 |
| 4 |
Byers, Caitlyn G (10)
|
MDMAC |
50.41 |
48.08 |
-2.33 |
| 5 |
Winters, Taylor L (10)
|
MDFSC |
50.99 |
48.12 |
-2.87 |
| 6 |
Rollins, Kylie M (10)
|
MDMAC |
51.36 |
48.48 |
-2.88 |
| 7 |
Mitchell, Ashley M (10)
|
MDMAS |
|
53.33 |
- |
| 8 |
Bronzi, Agnes R (10)
|
MDMAC |
1:02.54 |
58.90 |
-3.64 |
| 9 |
Price, Delaney G (10)
|
MDFAST |
|
1:04.62 |
- |
|
Varacalle-Goetz, Gina E (10)
|
MDHSST |
|
DQ |
- |
|
Mann, Grace E (10)
|
MDACA |
54.96 |
DQ |
- |
|
Addeh, Jasmine E (10)
|
MDMAC |
1:01.43 |
DQ |
- |