| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Harshman, Leah N (9)
|
MDFAST |
45.46 |
42.93 |
-2.53 |
| 2 |
Blaha, Sienna K (9)
|
MDEST |
43.29 |
44.24 |
0.95 |
| 3 |
Amoo, Kayla R (9)
|
MDMAC |
|
44.74 |
- |
| 4 |
Lawler, Samantha J (9)
|
MDFSC |
|
45.24 |
- |
| 5 |
Niles, Riley L (9)
|
MDEST |
45.70 |
45.52 |
-0.18 |
| 6 |
Morton, Za'kayah L (9)
|
MDBCSC |
|
46.31 |
- |
| 7 |
Hanlon, Kelsey R (9)
|
MDFSC |
47.21 |
46.88 |
-0.33 |
| 8 |
Kacher, Paige H (9)
|
MDACA |
46.09 |
47.36 |
1.27 |
| 9 |
Sobotka, Liliana R (9)
|
MDMAS |
50.05 |
50.21 |
0.16 |
| 10 |
Karanikolis, Eleni L (9)
|
MDEST |
|
51.56 |
- |
| 11 |
Foster, Siena R (9)
|
MDMAC |
52.97 |
52.70 |
-0.27 |
| 12 |
Gorgol, Madison E (9)
|
MDMAC |
53.94 |
57.19 |
3.25 |
| 13 |
Shaw, Rhiley I (9)
|
MDFMSC |
1:29.18 |
1:40.96 |
11.78 |
|
Johnson, Kayla N (9)
|
MDJCC |
|
DQ |
- |
|
Razunguzwa, Janelle N (9)
|
MDMAC |
|
DQ |
- |
|
Schwering, Olive D (9)
|
MDJCC |
|
DQ |
- |
|
Kostyukevich, Sofiya K (9)
|
MDACA |
|
DQ |
- |
|
Emerick, Claire R (9)
|
MDMAS |
53.78 |
NS |
- |