| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Austin W (8)
|
MDBCSC |
40.61 |
42.06 |
1.45 |
| 2 |
Mahoney, Brenden J (8)
|
MDNAAC |
48.12 |
45.06 |
-3.06 |
| 3 |
Ohtani, Haruki (7)
|
MDCAA |
|
46.02 |
- |
| 4 |
Rider, Nolan P (8)
|
MDNAAC |
58.01 |
47.48 |
-10.53 |
| 5 |
Douds, Dominic W (8)
|
MDFAST |
|
48.53 |
- |
| 6 |
McPeak, Liam J (8)
|
MDGTAC |
1:00.15 |
50.31 |
-9.84 |
| 7 |
Hammock, Jackson W (8)
|
MDMAC |
50.68 |
53.02 |
2.34 |
| 8 |
Gloss, John R (8)
|
MDMAC |
49.88 |
53.24 |
3.36 |
| 9 |
Cooper, Ryland A (8)
|
MDFMSC |
1:01.24 |
53.86 |
-7.38 |
| 10 |
Holt, Dylan T (8)
|
MDFSC |
1:09.92 |
55.57 |
-14.35 |
| 11 |
Ahn, Ryan Y (8)
|
MDCAA |
|
55.82 |
- |
| 12 |
Zemo, Samuel Y (8)
|
MDFSC |
55.77 |
55.99 |
0.22 |
| 13 |
Winokur, Maxwell R (7)
|
MDCGA |
54.10 |
56.32 |
2.22 |
| 14 |
Ademola, Oluwaseun J (8)
|
MDMAC |
1:16.66 |
1:05.72 |
-10.94 |
|
Torres-Naranjo, Samuel (7)
|
MDCGA |
|
DQ |
- |
|
Trotman, Liam C (6)
|
MDFMSC |
|
DQ |
- |
|
Porter, James E (8)
|
MDYCM |
|
DQ |
- |
|
Cason, Daawood (8)
|
MDBCSC |
|
NS |
- |