| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Scharper, Grace M (8)
|
MDNBAC |
40.32 |
40.19 |
-0.13 |
| 2 |
Xu, Sophia D (8)
|
MDEST |
43.32 |
40.38 |
-2.94 |
| 3 |
Powers, Addison R (8)
|
MDMAS |
49.47 |
43.41 |
-6.06 |
| 4 |
Nguyen, Megan M (8)
|
MDBAAC |
54.61 |
46.88 |
-7.73 |
| 5 |
Hilzendeger, Carmen S (8)
|
MDLBA |
47.20 |
48.14 |
0.94 |
| 6 |
DeLuca, Tessa E (8)
|
MDLBA |
44.96 |
50.71 |
5.75 |
| 7 |
Mackey, Allyson (Ally) C (8)
|
MDEST |
53.90 |
51.25 |
-2.65 |
| 8 |
Cheer, Cora (8)
|
MDCAA |
57.01 |
52.45 |
-4.56 |
| 9 |
Eyler, Maura B (8)
|
MDFSC |
55.05 |
52.58 |
-2.47 |
| 10 |
Starr, Amelia J (8)
|
MDGBSA |
|
53.32 |
- |
| 11 |
Olson, Alexis M (8)
|
MDMAC |
1:00.57 |
56.00 |
-4.57 |
| 12 |
Handwerger, Jenna E (8)
|
MDEST |
52.16 |
56.69 |
4.53 |
| 13 |
Annis, Eliza J (8)
|
MDLBA |
56.75 |
58.95 |
2.20 |
| 14 |
Conley, Kennedy M (8)
|
MDFAST |
59.40 |
59.16 |
-0.24 |
| 15 |
Boarman, Ann M (8)
|
MDEST |
|
59.23 |
- |
| 16 |
You, Emily P (8)
|
MDEST |
|
1:04.41 |
- |
| 17 |
Wollenweber, Olivia B (8)
|
MDEST |
|
1:06.21 |
- |
| 18 |
Silverman, Solona C (8)
|
MDMAS |
|
1:07.19 |
- |
|
Grimberg, Caroline E (8)
|
MDFAST |
|
DQ |
- |
|
Smith, Madison E (8)
|
MDEST |
55.63 |
NS |
- |