| # | Swimmer | Team | Seed | Time | Diff |
| 2 |
Sun, Sofie R (10)
|
MDEST |
|
41.58 |
- |
| 4 |
Marwaha, Sonia (10)
|
MDEST |
42.85 |
42.77 |
-0.08 |
| 7 |
Dengler, Emily C (10)
|
MDFSC |
48.78 |
43.72 |
-5.06 |
| 8 |
Pickering, Folasade A (10)
|
MDCAA |
47.39 |
44.53 |
-2.86 |
| 9 |
Shpritz, Annalyse O (10)
|
MDEST |
46.35 |
44.60 |
-1.75 |
| 17 |
Demory, Elizbabeth N (10)
|
MDFAST |
48.10 |
48.77 |
0.67 |
| 18 |
Kochunov, Anita P (10)
|
MDCAA |
47.84 |
48.82 |
0.98 |
| 19 |
Ivall, Olivia P (10)
|
MDFAST |
47.69 |
49.08 |
1.39 |
| 20 |
Curry, McKinley A (10)
|
MDBCSC |
58.07 |
49.79 |
-8.28 |
| 23 |
Hittle, Ava E (10)
|
MDEST |
|
59.05 |
- |
| 24 |
Raedeke, Brynn E (10)
|
MDHSST |
|
1:00.56 |
- |