| # | Swimmer | Team | Seed | Time | Diff |
| 2 |
Dragic, Nikola A (10)
|
MDFAST |
42.71 |
41.53 |
-1.18 |
| 3 |
Linstrom, Brian J (10)
|
MDCAA |
45.17 |
42.61 |
-2.56 |
| 4 |
Berrios, Kaito V (10)
|
MDEST |
44.53 |
43.83 |
-0.70 |
| 6 |
Pustavoitau, Zachary A (10)
|
MDEST |
43.13 |
44.06 |
0.93 |
| 7 |
Panfiorau, Anthony V (10)
|
MDEST |
44.14 |
45.58 |
1.44 |
| 8 |
Matthews, Kyle J (10)
|
MDFAST |
47.27 |
46.02 |
-1.25 |
| 9 |
Kirby, Tyler A (10)
|
MDHSST |
45.64 |
46.71 |
1.07 |
| 10 |
Ki, William J (10)
|
MDCAA |
|
48.21 |
- |
| 11 |
Dukes-Robinson, Christopher (10)
|
MDBCSC |
48.05 |
48.90 |
0.85 |
| 12 |
Roy, Rahul M (10)
|
MDEST |
50.13 |
52.92 |
2.79 |
| 13 |
Varacalle-Goetz, Eyan C (10)
|
MDHSST |
50.19 |
53.11 |
2.92 |
| 15 |
Davis, Andrew P (10)
|
MDFAST |
|
59.47 |
- |
| 16 |
Walcott, Myles M (10)
|
MDCAA |
1:07.66 |
59.96 |
-7.70 |
|
Allman, Luke R (10)
|
MDHSST |
49.09 |
NS |
- |