| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Austin W (8)
|
MDBCSC |
40.61 |
42.84 |
2.23 |
| 2 |
McDonnell, Victor T (8)
|
MDASC |
41.68 |
43.10 |
1.42 |
| 3 |
Goyette, Noah R (8)
|
MDASC |
45.39 |
46.84 |
1.45 |
| 4 |
McCallister, Prince K (8)
|
MDMAS |
48.65 |
47.76 |
-0.89 |
| 5 |
Wesby, Mitchell T (8)
|
MDMAS |
57.48 |
48.70 |
-8.78 |
| 6 |
Hart Jr, Matthew W (8)
|
MDLBA |
52.01 |
50.54 |
-1.47 |
| 7 |
Winters, Kyle D (8)
|
MDSPRC |
|
51.49 |
- |
| 8 |
Acchione, Joseph T (7)
|
MDLBA |
53.44 |
51.91 |
-1.53 |
| 9 |
Elliott Jr, Mark A (8)
|
MDEST |
|
52.40 |
- |
| 10 |
Holt, Dylan T (8)
|
MDFSC |
1:09.92 |
55.70 |
-14.22 |
| 11 |
Michalski, Nathaniel J (8)
|
MDSPRC |
1:04.20 |
1:02.94 |
-1.26 |
| 12 |
Bryant, Reid T (7)
|
MDCAC |
|
1:03.28 |
- |
| 13 |
Cason, Daawood (8)
|
MDBCSC |
|
1:10.61 |
- |