| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Homitz, Jessica L (16)
|
MASSC |
24.85 |
25.55 |
0.70 |
| 2 |
Chermol, Ashlyn M (18)
|
MASSC |
26.09 |
26.34 |
0.25 |
| 3 |
Mikhalev, Vitalina (15)
|
MACBST |
27.44 |
26.57 |
-0.87 |
| 4 |
Kosko, Catherine (15)
|
MATHY |
26.54 |
26.88 |
0.34 |
| 5 |
Edwards, Annika J (15)
|
MAFCA |
27.42 |
27.55 |
0.13 |
| 6 |
Campbell, Cailin E (15)
|
MAGSY |
27.20 |
27.80 |
0.60 |
| 7 |
Armetta, Karina (16)
|
MAGSY |
28.13 |
28.71 |
0.58 |
| 8 |
Worthington, Anna G (15)
|
MACBST |
29.62 |
30.38 |
0.76 |
| 9 |
Dietrich, Katherine A (16)
|
MAMSA |
30.08 |
30.81 |
0.73 |
|
Stephens, Abigail L (15)
|
MAAAC |
27.23 |
NS |
- |
|
Beno, Anna M (15)
|
MACBST |
25.04 |
NS |
- |
|
Olszewski, Elizabeth M (15)
|
MASSC |
27.59 |
NS |
- |
|
Nemceff, Christie M (16)
|
MATHY |
26.89 |
NS |
- |