| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Holley, Palmer S (12)
|
PVSDS |
33.54 |
31.74 |
-1.80 |
| 2 |
Vollbrecht, Sarah E (12)
|
PVTANK |
31.05 |
31.75 |
0.70 |
| 3 |
Nowak, Jessica G (12)
|
PVDCPR |
31.39 |
31.77 |
0.38 |
| 4 |
Fleck, Kristina S (11)
|
PVNCAP |
32.88 |
32.10 |
-0.78 |
| 5 |
Clark, Makenna (12)
|
PVDCPR |
33.43 |
32.21 |
-1.22 |
| 6 |
Yang, Julie (11)
|
PVNCAP |
32.80 |
33.20 |
0.40 |
| 7 |
Ipakchi, Ariana M (11)
|
PVNCAP |
34.68 |
35.41 |
0.73 |
| 8 |
Kumar, Neha (12)
|
PVDCPR |
33.91 |
35.84 |
1.93 |
| 9 |
Lewin, Cassidy P (11)
|
PVNCAP |
38.97 |
37.22 |
-1.75 |
| 10 |
Elmoutarajji, Ayah (11)
|
PVTANK |
38.41 |
39.13 |
0.72 |
| 11 |
Chloe, Sydney (11)
|
PVDCPR |
43.01 |
39.77 |
-3.24 |
| 12 |
Brinkley-Tyson, Malaijah A (11)
|
PVDCPR |
46.93 |
40.09 |
-6.84 |
| 13 |
Kay, Sandra P (11)
|
PVTANK |
39.94 |
40.24 |
0.30 |
| 14 |
Desel Dolan, Katherine M (12)
|
PVDCPR |
|
40.42 |
- |
| 15 |
Walker, Sydney L (11)
|
PVDCPR |
44.88 |
41.49 |
-3.39 |
| 16 |
Davis, Eva S (11)
|
PVDCPR |
|
44.74 |
- |
| 17 |
Shanks, Ava J (11)
|
PVDCPR |
58.25 |
45.36 |
-12.89 |
| 18 |
Manigo-Truell dos Sa, Do E (11)
|
PVDCPR |
|
47.46 |
- |
|
Padilla, Kayla (12)
|
PVDCPR |
37.23 |
NS |
- |