| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Larson, Kimberly P (23)
|
MRIA |
56.57 |
55.59 |
-0.98 |
| 2 |
Mackrel, Naima J (15)
|
MRNDAC |
56.06 |
59.30 |
3.24 |
| 3 |
Oyen-Ustad, Lillian E (17)
|
MRNDAC |
56.67 |
59.81 |
3.14 |
| 4 |
Brant, Eliza N (16)
|
MRIA |
58.75 |
59.93 |
1.18 |
| 5 |
Shiebler, Patricia E (15)
|
MRIA |
58.99 |
1:00.46 |
1.47 |
| 6 |
Gilio, Devin M (15)
|
MRIA |
1:03.31 |
1:00.60 |
-2.71 |
| 7 |
Regenauer, Emma A (17)
|
MRDA |
59.77 |
1:03.70 |
3.93 |
| 8 |
Sistare, Kathryn M (16)
|
MRNDAC |
1:00.50 |
1:03.99 |
3.49 |
| 9 |
Walsh, Katelyn (16)
|
MRPBAC |
1:06.46 |
1:10.05 |
3.59 |
| 10 |
Armstrong, Payton R (15)
|
MRMWSC |
1:10.39 |
1:10.20 |
-0.19 |