| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Morrison, Daphanie (10)
|
IAOTT |
34.90 |
33.74 |
-1.16 |
| 2 |
Miller, Kimberlee (10)
|
IANEWT |
33.48 |
35.16 |
1.68 |
| 3 |
Kettles, Tori E (10)
|
IADSMY |
36.06 |
36.94 |
0.88 |
| 4 |
Barton, Temple (9)
|
IANEWT |
35.84 |
37.91 |
2.07 |
| 5 |
Larsen, Faith (9)
|
IANVFY |
43.84 |
37.94 |
-5.90 |
| 6 |
Walter, Ella (10)
|
ITOSKY |
39.01 |
38.01 |
-1.00 |
| 7 |
Andriano, Natalie C (9)
|
IADSMY |
39.12 |
38.88 |
-0.24 |
| 8 |
Dunham, Keegan E (10)
|
IADSMY |
39.13 |
39.54 |
0.41 |
| 9 |
DePhillips, Samantha A (10)
|
IADSMY |
37.64 |
39.68 |
2.04 |
| 10 |
Manary, Kate (9)
|
IAOTT |
41.10 |
39.86 |
-1.24 |
| 11 |
Gustafson, Amanda K (10)
|
IADSMY |
43.88 |
39.99 |
-3.89 |
| 12 |
Hoffman, Molly A (10)
|
IADSMY |
44.19 |
40.62 |
-3.57 |
| 13 |
Molis, Ashley (10)
|
IADSMY |
42.40 |
40.64 |
-1.76 |
| 14 |
Shafer, Harper J (9)
|
IADSMY |
41.22 |
40.68 |
-0.54 |
| 15 |
Straw, Kaedyn M (9)
|
IADSMY |
45.26 |
40.87 |
-4.39 |
| 16 |
Duke, Mavis C (9)
|
IADSMY |
41.84 |
41.00 |
-0.84 |
| 17 |
Alexander, Jennifer O (9)
|
IADSMY |
42.05 |
41.43 |
-0.62 |
| 18 |
Dinkin, Kenzie E (10)
|
IADSMY |
43.98 |
42.04 |
-1.94 |
| 19 |
Samuelson, Lhotse M (10)
|
IADSMY |
42.38 |
42.39 |
0.01 |
| 20 |
Isenberg, Hayden L (9)
|
IADSMY |
48.99 |
43.02 |
-5.97 |
| 21 |
Palaparthy, Hiranmayi (10)
|
IADSMY |
46.27 |
45.29 |
-0.98 |
| 22 |
Grieshaber, Bailey (10)
|
IANVFY |
|
46.56 |
- |
| 23 |
Creger, Maya K (9)
|
IADSMY |
48.75 |
47.64 |
-1.11 |
| 24 |
Larson, Eleanor A (10)
|
IADSMY |
|
49.26 |
- |
| 25 |
Hoskins, Olivia (10)
|
IAOTT |
|
50.19 |
- |
| 26 |
Bhati, Drissya (9)
|
IADSMY |
|
50.27 |
- |
| 27 |
Messing, Surianna (10)
|
IANVFY |
|
56.03 |
- |
| 28 |
Umphress, Olivia A (9)
|
IADSMY |
|
1:03.50 |
- |
| 29 |
Hammontree, Hope (9)
|
IANVFY |
|
1:06.83 |
- |