| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Scales, Taylor A (10)
|
NICAGO |
50.26 |
47.61 |
-2.65 |
| 2 |
Janeski, Gabrielle M (10)
|
NIKRKN |
50.51 |
49.55 |
-0.96 |
| 3 |
Guild, Aleice E (10)
|
NIGGST |
48.63 |
49.60 |
0.97 |
| 4 |
Denlinger, Elizabeth J (9)
|
NIWAAC |
|
55.33 |
- |
| 5 |
Melveney, Emily R (9)
|
NIGGST |
|
55.77 |
- |
| 6 |
Pelletier, Eva U (10)
|
NIGGST |
1:01.46 |
57.69 |
-3.77 |
| 7 |
Kuksenkova, Maria M (9)
|
NIKRKN |
|
59.96 |
- |
| 8 |
Gonzalez, Hannah R (9)
|
NIKRKN |
1:06.68 |
1:13.26 |
6.58 |
| 9 |
Shutt, Halle E (9)
|
NIGVST |
|
1:21.77 |
- |
| 10 |
Sedlak, Eliza L (9)
|
NIKRKN |
|
1:25.98 |
- |
|
Zhou, Yuhan (9)
|
NIKVAC |
|
DQ |
- |
|
Peng, Cheryl S (9)
|
NIKRKN |
|
DQ |
- |
|
Wojciechowski, Ella G (9)
|
NIKRKN |
1:09.68 |
DQ |
- |