| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Browning, Cyrus M (15)
|
NIISWM |
49.33 |
49.64 |
0.31 |
| 2 |
McClelland, Caleb J (17)
|
NIWAAC |
50.47 |
50.79 |
0.32 |
| 3 |
Schultz, Michael (15)
|
NICVSC |
51.23 |
52.49 |
1.26 |
| 4 |
Tague, William F (17)
|
NIISWM |
53.37 |
52.67 |
-0.70 |
| 5 |
Anderson, Carter R (15)
|
NIISWM |
51.85 |
52.77 |
0.92 |
| 6 |
Bernhardt, Hunter M (17)
|
NICORY |
54.61 |
54.30 |
-0.31 |
| 7 |
Silbert, Benjamin N (15)
|
NIISWM |
55.72 |
55.03 |
-0.69 |
| 8 |
Williamson, Ethan L (18)
|
NIKVAC |
53.90 |
55.09 |
1.19 |
| 9 |
Nached, Greg M (15)
|
NIKRKN |
54.01 |
55.13 |
1.12 |
| 10 |
Perry, Westin C (16)
|
NIKVAC |
55.45 |
55.52 |
0.07 |
| 11 |
McClelland, Joshua W (16)
|
NIWAAC |
1:11.10 |
56.20 |
-14.90 |
| 12 |
Quintana, Lucas (15)
|
NIKVAC |
59.10 |
58.39 |
-0.71 |
| 13 |
Capozzi, Anthony E (15)
|
NISTSC |
59.00 |
59.94 |
0.94 |
| 14 |
Dongtoe, Tenzin C (18)
|
NIISWM |
1:03.34 |
1:00.49 |
-2.85 |
| 15 |
Greenfield, Zachary P (15)
|
NIKVAC |
1:02.36 |
1:03.09 |
0.73 |
| 16 |
Lane, Martin D (57)
|
NIWAAC |
1:08.87 |
1:12.08 |
3.21 |