| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Moody, Claire V (14)
|
VAGATR |
30.20 |
28.85 |
-1.35 |
| 2 |
Burr, Kathryn A (14)
|
VACYAC |
28.68 |
29.15 |
0.47 |
| 3 |
Vanyo, Athena A (13)
|
VACYAC |
28.76 |
29.23 |
0.47 |
| 4 |
Harlow, Abigayle A (14)
|
VAGATR |
29.11 |
29.43 |
0.32 |
| 5 |
Haise, Sophie A (14)
|
VACYAC |
29.12 |
29.88 |
0.76 |
| 6 |
O'Donnell, Sophie K (14)
|
VACYAC |
31.00 |
30.24 |
-0.76 |
| 7 |
Niculescu, Gabriela E (13)
|
VASMAC |
31.00 |
30.54 |
-0.46 |
| 8 |
Smith, Virginia E (13)
|
VACYAC |
33.23 |
30.63 |
-2.60 |
| 9 |
Murie, Rachel H (14)
|
VASMAC |
29.68 |
30.73 |
1.05 |
| 10 |
Barlow, Naska M (14)
|
VARACE |
36.29 |
33.01 |
-3.28 |
| 11 |
Farabaugh, Kristen N (13)
|
VACYAC |
35.56 |
36.71 |
1.15 |
| 12 |
Elkins, Alice A (13)
|
VASMAC |
34.69 |
37.18 |
2.49 |
| 13 |
Mundie, Reagan O (13)
|
VASMAC |
|
44.40 |
- |