| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wells, Madelyn I (13)
|
VAGATR |
39.61 |
39.33 |
-0.28 |
| 2 |
Hanstedt, Lucinda E (14)
|
VASTRM |
|
41.17 |
- |
| 2 |
Tosi, Arianna L (14)
|
VAGATR |
50.24 |
41.17 |
-9.07 |
| 4 |
Farabaugh, Kristen N (14)
|
VACYAC |
44.21 |
42.93 |
-1.28 |
| 5 |
Czirjak, Evelyn M (13)
|
VAGATR |
|
43.40 |
- |
| 6 |
Gibbons, Delaney F (13)
|
VA757 |
52.68 |
43.98 |
-8.70 |
| 7 |
Hill, Sydney G (13)
|
VASMAC |
47.73 |
44.33 |
-3.40 |
| 8 |
Niculescu, Gabriela E (14)
|
VASMAC |
45.88 |
44.47 |
-1.41 |
| 9 |
Copening, Emma G (14)
|
VASCAT |
43.14 |
45.40 |
2.26 |
| 10 |
Schwarzmann, Heidi J (13)
|
VASTRM |
|
51.46 |
- |
| 11 |
Smith, Julia D (13)
|
VASMAC |
57.50 |
56.27 |
-1.23 |
|
Alley, Matilda M (14)
|
VARACE |
|
NS |
- |