| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gibbons, Delaney F (14)
|
VA757 |
2:38.69 |
2:45.65 |
6.96 |
| 2 |
McLaughlin, Delaney L (13)
|
VACCA |
2:49.93 |
2:46.35 |
-3.58 |
| 3 |
Thompson, Eliza G (14)
|
VAGATR |
2:51.54 |
2:49.04 |
-2.50 |
| 4 |
Breeden, Emily F (13)
|
VASMAC |
2:54.67 |
2:49.05 |
-5.62 |
| 5 |
Lough, Katelyn A (13)
|
VAGATR |
2:42.51 |
2:49.40 |
6.89 |
| 6 |
Alms, Melissa N (13)
|
VAGATR |
|
2:49.47 |
- |
| 7 |
Reed, Shelby E (13)
|
VA757 |
2:46.53 |
2:50.72 |
4.19 |
| 8 |
Malone, Madeline N (14)
|
VAGATR |
3:10.70 |
2:51.59 |
-19.11 |
| 9 |
Deeble, Zoe E (13)
|
VAGATR |
2:44.38 |
2:52.99 |
8.61 |
| 10 |
Gorby, Amelia G (13)
|
VAGATR |
2:49.10 |
2:53.03 |
3.93 |
| 11 |
Kullander, Abigail L (13)
|
VAGATR |
2:49.18 |
3:01.06 |
11.88 |
| 12 |
Weaber, Anna D (13)
|
VASMAC |
3:03.12 |
3:04.68 |
1.56 |
| 13 |
Blair, Naomi L (13)
|
VASMAC |
3:15.79 |
3:11.21 |
-4.58 |
| 14 |
Parker, Julia P (14)
|
VACCA |
3:31.17 |
3:23.83 |
-7.34 |
|
Couric, Lydia W (13)
|
VAGATR |
2:45.40 |
NS |
- |
|
Yeroian, Anna J (13)
|
VAGATR |
3:22.08 |
NS |
- |