| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Vanyo, Sofie N (15)
|
VAACAC |
36.01 |
35.32 |
-0.69 |
| 2 |
Barcia, Caroline E (13)
|
VACA-Y |
37.43 |
35.63 |
-1.80 |
| 3 |
Mahland, Kaelyn M (14)
|
VALAC |
38.59 |
38.63 |
0.04 |
| 4 |
Heale, Annie (15)
|
VAACAC |
39.70 |
40.35 |
0.65 |
| 5 |
Gorby, Tessa L (17)
|
VACCA |
|
40.41 |
- |
| 6 |
Neal, Aislynn L (14)
|
VASMAC |
43.90 |
41.73 |
-2.17 |
| 7 |
Heale, Emmeline (13)
|
VAACAC |
40.93 |
42.84 |
1.91 |
| 8 |
James, Alyssa R (14)
|
VARCR |
44.34 |
43.52 |
-0.82 |
| 9 |
Jones, Layne C (17)
|
VACCA |
48.99 |
44.26 |
-4.73 |
| 10 |
Stisser, Evelyn D (13)
|
VASMAC |
44.65 |
44.44 |
-0.21 |
| 11 |
White, Jakiah I (15)
|
VASMAC |
48.41 |
45.67 |
-2.74 |
| 12 |
Heletz, Eve B (14)
|
VACCA |
|
45.84 |
- |
| 13 |
Dolinger, Delila G (13)
|
VACA-Y |
47.74 |
47.27 |
-0.47 |
| 14 |
Stevens, Kirsten M (17)
|
VAACAC |
49.46 |
49.51 |
0.05 |
| 15 |
Petrikonis, Rome A (13)
|
VALAC |
52.88 |
53.52 |
0.64 |
| 16 |
Fink, Eliza G (13)
|
VALAC |
50.28 |
54.18 |
3.90 |
| 17 |
Longmire, Clara (17)
|
VASMAC |
|
55.87 |
- |
| 18 |
Miller, Julia Z (17)
|
VASMAC |
55.77 |
56.00 |
0.23 |
| 19 |
Moyer, Eva L (13)
|
VARCR |
1:01.07 |
56.42 |
-4.65 |
| 20 |
McCatty, Camden I (14)
|
VARCR |
57.30 |
58.72 |
1.42 |
| 21 |
White, Jaliyah E (14)
|
VASMAC |
53.44 |
58.75 |
5.31 |
| 22 |
Allen, Tianna C (14)
|
WVGVAC |
1:06.28 |
1:02.18 |
-4.10 |
|
Melton, Madeline B (15)
|
VASMAC |
1:07.77 |
NS |
- |
|
Pullen, Alexis L (15)
|
VASMAC |
45.02 |
NS |
- |
|
Reichardt, Caroline N (17)
|
VACCA |
|
NS |
- |
|
Keller, Madison G (16)
|
VAGATR |
43.14 |
NS |
- |