| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Haise, Sophie A (14)
|
VACYAC |
35.09 |
34.06 |
-1.03 |
| 2 |
Moody, Claire V (14)
|
VAGATR |
33.94 |
34.07 |
0.13 |
| 3 |
Harlow, Abigayle A (14)
|
VAGATR |
35.81 |
35.20 |
-0.61 |
| 4 |
Niculescu, Gabriela E (13)
|
VASMAC |
37.31 |
36.73 |
-0.58 |
| 5 |
Blaha, Kayla A (14)
|
VARACE |
39.17 |
38.20 |
-0.97 |
| 6 |
Hanstedt, Lucinda E (13)
|
VASTRM |
39.75 |
39.01 |
-0.74 |
| 7 |
Smith, Virginia E (13)
|
VACYAC |
42.28 |
39.25 |
-3.03 |
| 8 |
Perry, Aldaynsh H (14)
|
VARACE |
36.39 |
40.10 |
3.71 |
| 9 |
Vaught, Ella G (13)
|
VASTRM |
46.35 |
43.38 |
-2.97 |
| 10 |
Farabaugh, Kristen N (13)
|
VACYAC |
46.72 |
43.66 |
-3.06 |
| 11 |
Elkins, Alice A (13)
|
VASMAC |
41.19 |
44.20 |
3.01 |
| 12 |
Mundie, Reagan O (13)
|
VASMAC |
|
45.31 |
- |