| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wagner, Kristen L (17)
|
VASMAC |
32.87 |
33.55 |
0.68 |
| 2 |
Sullivan, Kaitlyn C (15)
|
VA757 |
35.65 |
33.90 |
-1.75 |
| 3 |
Long, Sofia R (17)
|
VA757 |
34.84 |
33.98 |
-0.86 |
| 4 |
Moody, Claire V (16)
|
VAGATR |
33.94 |
34.24 |
0.30 |
| 5 |
Nardone, Stephanie M (20)
|
VAGATR |
34.18 |
35.24 |
1.06 |
| 6 |
Wyatt, Elizabeth (16)
|
VAGATR |
38.15 |
36.61 |
-1.54 |
| 7 |
Tosi, Arianna L (15)
|
VAGATR |
37.72 |
36.66 |
-1.06 |
| 8 |
Arndt, Olivia D (17)
|
VAGATR |
38.11 |
36.67 |
-1.44 |
| 9 |
Kashatus, Audrey E (16)
|
VAGATR |
34.83 |
36.80 |
1.97 |
| 10 |
Kastner, Niamh C (15)
|
VA757 |
37.02 |
36.90 |
-0.12 |
| 11 |
Flanagan, Lainey R (16)
|
VA757 |
36.75 |
37.14 |
0.39 |
| 12 |
Lynch, Virginia N (17)
|
VA757 |
37.01 |
37.19 |
0.18 |
| 13 |
Arnold, Sarah E (17)
|
VAGATR |
34.97 |
37.21 |
2.24 |
| 14 |
Johnson, Kylie M (17)
|
VA757 |
36.58 |
37.30 |
0.72 |
| 15 |
Bendall, Elisabeth A (15)
|
VAGATR |
39.19 |
37.38 |
-1.81 |
| 16 |
Copening, Emma G (15)
|
VASCAT |
39.57 |
38.78 |
-0.79 |
| 17 |
Batista, Anna C (15)
|
VACCA |
|
41.65 |
- |
| 18 |
Martin, Avery L (15)
|
VA757 |
|
41.82 |
- |
| 19 |
Sulanke, Claire E (15)
|
VARACE |
40.00 |
42.73 |
2.73 |
| 20 |
Pietrowski, Anja J (15)
|
VASMAC |
|
46.73 |
- |
| 21 |
Miller, Madeline E (15)
|
VASCAT |
|
52.07 |
- |