| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Oswald, Aiden C (12)
|
VAGATR |
48.54 |
41.15 |
-7.39 |
| 2 |
He, Quinn T (12)
|
VAGATR |
45.21 |
43.57 |
-1.64 |
| 3 |
Ritter, Beckett D (11)
|
VAGATR |
55.97 |
43.92 |
-12.05 |
| 4 |
McMahon, III, Robert W (12)
|
VAGATR |
44.02 |
44.11 |
0.09 |
| 5 |
Yang, Chenghao (12)
|
VAGATR |
47.41 |
46.72 |
-0.69 |
| 6 |
Bowman, Henry E (11)
|
VAGATR |
|
49.61 |
- |
| 7 |
Maust, Samuel L (11)
|
VAGATR |
|
50.38 |
- |
| 8 |
Weaber, Anderson E (11)
|
VAGATR |
56.63 |
50.42 |
-6.21 |
| 9 |
Tungate, Maxswell L (11)
|
VAGATR |
|
51.08 |
- |
| 10 |
Straight, Christian B (12)
|
VAGATR |
49.59 |
51.36 |
1.77 |
| 11 |
Dmytriyev, Boris (11)
|
VAGATR |
54.79 |
56.03 |
1.24 |
| 12 |
Fish, Xavier L (12)
|
VAGATR |
1:03.57 |
56.08 |
-7.49 |