| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Long, Sofia R (16)
|
VA757 |
2:33.63 |
2:33.12 |
-0.51 |
| 2 |
Blom, Brooke S (15)
|
WVWILD |
2:50.21 |
2:40.23 |
-9.98 |
| 3 |
Larson, Abigail R (18)
|
VA757 |
2:31.56 |
2:41.21 |
9.65 |
| 4 |
Wagner, Kristen L (16)
|
VASMAC |
2:41.20 |
2:42.67 |
1.47 |
| 5 |
Johnson, Kylie M (16)
|
VA757 |
2:32.15 |
2:43.12 |
10.97 |
| 6 |
Lynch, Virginia N (16)
|
VA757 |
2:37.64 |
2:44.45 |
6.81 |
| 7 |
Raines, Regan E (18)
|
WVWILD |
2:34.86 |
2:48.13 |
13.27 |
| 8 |
Gross, Bojena D (17)
|
WVWILD |
2:53.32 |
3:01.20 |
7.88 |
| 9 |
Humphreys, Samantha G (16)
|
WVWILD |
2:54.87 |
3:03.43 |
8.56 |
| 10 |
Hill, Emma K (17)
|
VASMAC |
2:59.84 |
3:04.35 |
4.51 |
| 11 |
Fitzgerald, McKina C (18)
|
VASMAC |
2:55.41 |
3:04.60 |
9.19 |
| 12 |
Rowe, Erin (16)
|
WVWILD |
3:03.32 |
3:38.72 |
35.40 |
|
Breza, Morgan M (15)
|
VACYAC |
2:53.53 |
NS |
- |
|
Weathersby, Maren C (17)
|
VACYAC |
2:33.65 |
NS |
- |