| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Weaber, Anderson E (12)
|
VASMAC |
32.39 |
32.50 |
0.11 |
| 2 |
Yang, Chenghao (12)
|
VAGATR |
38.17 |
34.73 |
-3.44 |
| 3 |
Maust, Samuel L (11)
|
VAGATR |
35.12 |
35.38 |
0.26 |
| 4 |
Rockwell, Thomas D (11)
|
VASMAC |
39.97 |
36.32 |
-3.65 |
| 5 |
He, Quinn T (12)
|
VASMAC |
39.95 |
37.02 |
-2.93 |
| 6 |
Straight, Christian B (12)
|
VASMAC |
37.47 |
37.90 |
0.43 |
| 7 |
Xie, William (11)
|
VAGATR |
41.52 |
39.21 |
-2.31 |
| 8 |
Kasten, Francis E (12)
|
VAGATR |
1:01.30 |
40.89 |
-20.41 |
| 9 |
Kerrigan, Tyler W (11)
|
VAGATR |
43.51 |
40.90 |
-2.61 |
| 10 |
Apicella, Ethan S (11)
|
VASMAC |
41.80 |
41.41 |
-0.39 |
| 11 |
Alhusen, Maximilian K (12)
|
VASMAC |
|
42.01 |
- |
| 12 |
Coleman, Joseph J (12)
|
VAGATR |
47.64 |
42.11 |
-5.53 |
| 13 |
Thomas, Caleb P (12)
|
VAGATR |
52.72 |
48.34 |
-4.38 |
| 14 |
Patel, Shane D (11)
|
VASMAC |
|
51.96 |
- |
|
Milam, Jeb P (11)
|
VALAC |
39.76 |
NS |
- |