| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Long, Sofia R (17)
|
VA757 |
30.65 |
30.80 |
0.15 |
| 2 |
Gusler, Lily K (17)
|
VAGATR |
29.68 |
31.14 |
1.46 |
| 3 |
Sullivan, Kaitlyn C (15)
|
VA757 |
34.19 |
31.16 |
-3.03 |
| 4 |
Nardone, Stephanie M (20)
|
VAGATR |
|
31.38 |
- |
| 5 |
McCay, Madeleine H (15)
|
VAGATR |
31.13 |
31.73 |
0.60 |
| 6 |
Wagner, Kristen L (17)
|
VASMAC |
31.52 |
31.99 |
0.47 |
| 7 |
Lynch, Virginia N (17)
|
VA757 |
33.00 |
33.30 |
0.30 |
| 8 |
Johnson, Keira L (16)
|
VAGATR |
37.22 |
33.77 |
-3.45 |
| 9 |
Bendall, Elisabeth A (15)
|
VAGATR |
41.34 |
33.89 |
-7.45 |
| 10 |
Flanagan, Lainey R (16)
|
VA757 |
41.75 |
33.92 |
-7.83 |
| 11 |
Kastner, Niamh C (15)
|
VA757 |
34.13 |
34.08 |
-0.05 |
| 12 |
Johnson, Kylie M (17)
|
VA757 |
34.32 |
35.05 |
0.73 |
| 13 |
Kashatus, Audrey E (16)
|
VAGATR |
32.37 |
35.40 |
3.03 |
| 14 |
Arnold, Sarah E (17)
|
VAGATR |
32.74 |
35.48 |
2.74 |
| 15 |
Copening, Emma G (15)
|
VASCAT |
33.90 |
35.77 |
1.87 |
| 16 |
Wyatt, Elizabeth (16)
|
VAGATR |
35.00 |
37.23 |
2.23 |
| 17 |
Martin, Avery L (15)
|
VA757 |
|
40.65 |
- |
| 18 |
Batista, Anna C (15)
|
VACCA |
|
41.28 |
- |
| 19 |
Pietrowski, Anja J (15)
|
VASMAC |
|
43.90 |
- |
|
Moody, Claire V (16)
|
VAGATR |
32.03 |
NS |
- |
|
Tosi, Arianna L (15)
|
VAGATR |
39.16 |
NS |
- |
|
Tosi, Gabriella M (19)
|
VAGATR |
35.03 |
NS |
- |