| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Baley, Ava C (9)
|
NCAJCC |
41.54 |
39.52 |
-2.02 |
| 2 |
Jenkins, Charlotte F (10)
|
NCAJCC |
42.04 |
40.75 |
-1.29 |
| 3 |
Savage, Mary Jane M (9)
|
NCAJCC |
42.31 |
41.67 |
-0.64 |
| 4 |
Hardesty, Sloane (10)
|
NCAJCC |
44.41 |
43.68 |
-0.73 |
| 5 |
Carver, Isla R (9)
|
NCSMAC |
43.36 |
43.71 |
0.35 |
| 6 |
Mason, Ella M (9)
|
NCSMAC |
47.63 |
45.58 |
-2.05 |
| 7 |
Sanders, Hadley E (10)
|
SEACAC |
50.52 |
46.20 |
-4.32 |
| 8 |
McGowin, Casey E (9)
|
NCBAQ |
58.14 |
49.20 |
-8.94 |
| 9 |
Springer, Annette J (10)
|
SEACAC |
58.84 |
55.79 |
-3.05 |
| 10 |
Galindo-Gomez, Jayleen A (10)
|
NCSMAC |
58.04 |
56.48 |
-1.56 |
| 11 |
Adams, Emery L (9)
|
NCSMAC |
54.02 |
59.14 |
5.12 |
|
Carr, Emma M (10)
|
NCMHAC |
48.73 |
DQ |
- |