| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Von Holtz, Alexa L (12)
|
NIMETS |
5:04.20 |
4:41.04 |
-23.16 |
| 2 |
Lee, Sophia (11)
|
NIUN-D |
4:47.94 |
4:48.26 |
0.32 |
| 3 |
Xu, Ming Na (12)
|
NIFAST |
5:07.59 |
5:08.93 |
1.34 |
| 4 |
Reilly, Fiona K (12)
|
NISTAR |
5:20.11 |
5:13.12 |
-6.99 |
| 5 |
Pirigyi, Sophia J (12)
|
NISTAR |
5:30.99 |
5:19.77 |
-11.22 |
| 6 |
Barros, Rachel (11)
|
NIUN-B |
5:30.68 |
5:21.47 |
-9.21 |
| 7 |
Guagliardo, Giovanna (12)
|
NIIRON |
5:39.48 |
5:26.50 |
-12.98 |
| 8 |
Caldwell, Hannah M (12)
|
NIVICT |
5:45.86 |
5:26.56 |
-19.30 |
| 9 |
Berdysiak, Elizabeth C (12)
|
NICLSC |
5:40.87 |
5:35.45 |
-5.42 |
| 10 |
Beck, Jenna R (11)
|
NICVSC |
5:41.97 |
5:39.26 |
-2.71 |
| 11 |
Zawodzinski, Zoe E (12)
|
NIHAMB |
5:38.71 |
5:41.36 |
2.65 |
|
McKinney, Madison N (11)
|
NICLSC |
5:44.07 |
DQ |
- |
|
Shen, Alivia (11)
|
NIPACK |
5:15.01 |
DQ |
- |