| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Weber, Sophia R (8)
|
NIWEBS |
21.46 |
20.76 |
-0.70 |
| 2 |
Uchal, Lilah A (7)
|
NIWEBS |
21.42 |
21.25 |
-0.17 |
| 3 |
Nichols, Maria Ada G (8)
|
NIPACK |
22.05 |
22.32 |
0.27 |
| 4 |
Mantell, Julia R (7)
|
NIIRON |
22.29 |
23.41 |
1.12 |
| 4 |
Orlando, Caralynn R (8)
|
NIPACK |
22.12 |
23.41 |
1.29 |
| 6 |
Ritter, Catherine (7)
|
NIPACK |
25.27 |
24.13 |
-1.14 |
| 7 |
Gurney, Allison A (8)
|
NIOLSC |
26.30 |
25.36 |
-0.94 |
| 8 |
Geary, Emma K (6)
|
NIMERC |
25.04 |
25.70 |
0.66 |
| 9 |
Hershey, Elianna N (8)
|
NIWEBS |
27.09 |
26.35 |
-0.74 |
| 10 |
Burke, Alexandra L (8)
|
NIPACK |
25.81 |
26.69 |
0.88 |
| 11 |
Danforth, Mckenna R (7)
|
NIWEBS |
26.39 |
26.98 |
0.59 |
| 12 |
Zappia, Adrienne H (8)
|
NIPACK |
26.13 |
27.27 |
1.14 |
| 13 |
Wojieck, Teagan E (8)
|
NIWGSC |
26.59 |
27.37 |
0.78 |
| 14 |
Frenzel, Lillian N (7)
|
NIWEBS |
25.70 |
27.48 |
1.78 |
| 15 |
Garigen, Molly G (7)
|
NIWEBS |
27.89 |
28.68 |
0.79 |
| 16 |
Reed, Alyssa M (8)
|
NIWGSC |
27.13 |
29.81 |
2.68 |
| 17 |
Murray, Mary Claire D (7)
|
NIWEBS |
32.12 |
29.87 |
-2.25 |
| 18 |
Smykla, Raegan D (8)
|
NIGRSC |
31.65 |
30.59 |
-1.06 |
| 19 |
Juby, Gracyn M (7)
|
NIWEBS |
35.00 |
35.39 |
0.39 |
|
Campanella, Alexandra P (8)
|
NIWEBS |
31.00 |
NS |
- |