| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Walsh, Alexandra (12)
|
CTCT |
55.31 |
55.38 |
0.07 |
| 2 |
Fieeiki, Charlotte L (12)
|
PVPV |
57.88 |
58.29 |
0.41 |
| 3 |
Mack, Katelyn G (12)
|
PVPV |
57.35 |
58.38 |
1.03 |
| 4 |
Buerger, Victoria H (12)
|
AMAM |
1:00.15 |
59.93 |
-0.22 |
| 5 |
Redican, Anna S (12)
|
PVPV |
1:00.25 |
1:00.35 |
0.10 |
| 6 |
Hardy, Sarah (12)
|
NJNJS |
59.80 |
1:00.73 |
0.93 |
| 7 |
Comaniciu, Alexandra (12)
|
NJNJS |
1:01.72 |
1:01.56 |
-0.16 |
| 8 |
Kaufmann, Noelle E (12)
|
MRMETR |
1:01.75 |
1:01.82 |
0.07 |
| 9 |
Raab, Shannon C (12)
|
MAMA |
1:01.08 |
1:01.92 |
0.84 |
| 10 |
Scott, Samantha (12)
|
CTCT |
1:02.17 |
1:02.39 |
0.22 |
| 11 |
Hu, Danning A (12)
|
NJNJS |
1:01.11 |
1:02.55 |
1.44 |
| 12 |
Silveira, Luiza E (12)
|
MRMETR |
1:00.78 |
1:02.78 |
2.00 |
| 13 |
McCune, Allison G (12)
|
NINI |
1:04.26 |
1:03.09 |
-1.17 |
| 14 |
Pentrack, Emma R (12)
|
AMAM |
1:02.37 |
1:03.31 |
0.94 |
| 15 |
Fraas, Mia (12)
|
CTCT |
1:02.94 |
1:03.64 |
0.70 |
| 16 |
Goettler, Laura B (12)
|
AMAM |
1:02.41 |
1:03.81 |
1.40 |
| 17 |
Regenauer, Christiana M (12)
|
MRMETR |
1:00.87 |
1:04.01 |
3.14 |
| 17 |
Kolessar, Madison M (11)
|
MAMA |
1:02.72 |
1:04.01 |
1.29 |
| 19 |
Williams, Mattie B (12)
|
ADAD |
1:03.99 |
1:04.45 |
0.46 |
| 20 |
Carper, Ashley N (12)
|
MAMA |
1:02.32 |
1:04.68 |
2.36 |
| 21 |
Derosia, Chloe E (12)
|
ADAD |
1:06.20 |
1:04.86 |
-1.34 |
| 22 |
Pierce, Summer M (12)
|
NINI |
1:03.71 |
1:05.71 |
2.00 |
| 23 |
Ritz, Erin (12)
|
NINI |
1:05.09 |
1:06.16 |
1.07 |
| 24 |
Hamilton, Sidney C (12)
|
MEMAIN |
1:06.35 |
1:06.46 |
0.11 |
| 25 |
Hartwich, Alexandra G (12)
|
ADAD |
1:05.82 |
1:06.79 |
0.97 |
| 26 |
McLeod, Marjorie E (11)
|
MEMAIN |
1:07.20 |
1:08.58 |
1.38 |
| 27 |
Doughty, Emma J (12)
|
NINI |
1:07.28 |
1:10.10 |
2.82 |
| 28 |
Mahoney, Caroline E (12)
|
MEMAIN |
1:07.03 |
1:11.16 |
4.13 |