| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Witte, Dana C (14)
|
NIWEBS |
25.46 |
25.39 |
-0.07 |
| 1 |
Jones, Katherine E (14)
|
NITWST |
24.57 |
25.39 |
0.82 |
| 3 |
Worrall, Alexis R (14)
|
NIFAST |
27.28 |
26.21 |
-1.07 |
| 4 |
Switalski, Alexandra A (14)
|
NITTSC |
26.87 |
26.56 |
-0.31 |
| 5 |
Hahn, Elizabeth P (14)
|
NIWEBS |
25.07 |
26.71 |
1.64 |
| 6 |
Ritter, Anne (14)
|
NIPACK |
26.78 |
27.10 |
0.32 |
| 7 |
Chugh, Katie F (14)
|
NIEAST |
27.23 |
27.67 |
0.44 |
| 8 |
Wood, Mya E (14)
|
NITWST |
27.03 |
27.87 |
0.84 |
| 9 |
Forcucci, Angela (14)
|
NITWST |
27.38 |
27.89 |
0.51 |
| 10 |
Flash, Maya I (14)
|
NITTSC |
27.62 |
28.09 |
0.47 |
| 11 |
Taberski, Kathleen M (14)
|
NISDSC |
28.24 |
28.26 |
0.02 |
| 12 |
Marshall, Olivia J (14)
|
NIEMAC |
28.34 |
28.54 |
0.20 |
| 13 |
Panzica, Elizabeth N (14)
|
NITTSC |
28.67 |
28.56 |
-0.11 |
| 14 |
Musterait, Madeline A (14)
|
NITWST |
28.73 |
28.95 |
0.22 |
| 15 |
Patterson, Ellen R (14)
|
NIIRON |
28.76 |
29.73 |
0.97 |
| 16 |
von Holtz, Reatha E (14)
|
NIMETS |
27.26 |
29.80 |
2.54 |
| 17 |
Miterko, Hannah C (14)
|
NITWST |
28.39 |
30.61 |
2.22 |