| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
El Ghissassi, Ritaj (11)
|
NIUN-N |
32.97 |
34.28 |
1.31 |
| 2 |
Guilfoil, Ella K (12)
|
NIFAST |
35.19 |
34.48 |
-0.71 |
| 3 |
Xu, Ming Na (11)
|
NIFAST |
35.66 |
35.64 |
-0.02 |
| 4 |
Solodova, Elena A (12)
|
NIFAST |
37.72 |
37.02 |
-0.70 |
| 5 |
Curry, Morgan (11)
|
NISEYS |
37.22 |
39.07 |
1.85 |
| 6 |
Guagliardo, Giovanna (11)
|
NIIRON |
|
39.08 |
- |
| 7 |
Shen, Alivia (10)
|
NIPACK |
43.43 |
39.98 |
-3.45 |
| 8 |
Jefferis, Ilah M (12)
|
NIISWM |
44.82 |
41.32 |
-3.50 |
| 9 |
Aitchison, Jocelyn C (11)
|
NISEYS |
38.11 |
41.55 |
3.44 |
| 10 |
Gorton, Claire P (11)
|
NIWEBS |
56.33 |
42.83 |
-13.50 |
| 11 |
Black, Meghan C (12)
|
NIFAST |
54.94 |
44.44 |
-10.50 |
| 12 |
Goodreau, Josephine M (12)
|
NIISWM |
50.59 |
46.75 |
-3.84 |
| 13 |
Headrick, Skyler F (12)
|
NIISWM |
54.66 |
46.85 |
-7.81 |
| 14 |
Guo, Angie Y (12)
|
NIISWM |
59.08 |
49.78 |
-9.30 |
| 15 |
Magnera, Sofia A (11)
|
NIWEBS |
56.17 |
50.32 |
-5.85 |
| 16 |
Polito, Clara T (11)
|
NIQUIK |
48.19 |
51.31 |
3.12 |
| 17 |
Beutner, Ella R (11)
|
NIQUIK |
|
53.64 |
- |
| 18 |
Robinson, Sara C (11)
|
NISDSC |
|
54.46 |
- |
| 19 |
Gilbert, Natalie J (8)
|
NIQUIK |
|
57.53 |
- |
| 20 |
Nguessamble, Tristyn D (11)
|
NISDSC |
|
1:03.35 |
- |
| 21 |
Crowley, Lila M (8)
|
NIGRSC |
|
1:09.37 |
- |
|
Buck, Kathryn E (11)
|
NIWEBS |
|
NS |
- |
|
Williams, Tory L (12)
|
NIPACK |
59.07 |
NS |
- |