| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nguyen, Vivian A (12)
|
NIWEBS |
2:30.00 |
2:29.49 |
-0.51 |
| 2 |
Guagliardo, Giovanna (12)
|
NIIRON |
2:35.61 |
2:36.65 |
1.04 |
| 3 |
Onze, Lauren W (11)
|
NIVICT |
3:23.19 |
2:52.29 |
-30.90 |
| 4 |
Spencer, Elyse (11)
|
NIAMTS |
3:04.73 |
2:52.73 |
-12.00 |
| 5 |
Wang, Floria M (11)
|
NIFAST |
3:03.38 |
2:57.34 |
-6.04 |
| 6 |
Hersh, Erin (11)
|
NIVICT |
3:15.89 |
2:58.74 |
-17.15 |
| 7 |
Resch, Rebecka J (11)
|
NIGRSC |
3:07.25 |
3:19.14 |
11.89 |
| 8 |
VanDamme, Avery M (12)
|
NIWEBS |
3:30.00 |
3:26.35 |
-3.65 |
| 9 |
Stefanou, Ava C (12)
|
NIQUIK |
3:37.50 |
3:37.67 |
0.17 |
|
Huerta-Andrade, Isabella (12)
|
NIPACK |
2:47.14 |
NS |
- |