| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Evans, Rebecca L (16)
|
NIFAST |
5:16.11 |
5:32.91 |
16.80 |
| 2 |
Viola, Serafina C (14)
|
NIFAST |
5:49.82 |
5:52.21 |
2.39 |
| 3 |
Wolcott, Payton R (16)
|
NIGRSC |
5:40.66 |
5:59.45 |
18.79 |
| 4 |
Baggett, Mia A (16)
|
NIFAST |
5:44.76 |
6:05.15 |
20.39 |
| 5 |
Nenadic, Maria G (15)
|
NIFAST |
5:37.02 |
6:13.63 |
36.61 |
| 6 |
Federico, Sydney A (15)
|
NIGRSC |
6:31.59 |
6:16.69 |
-14.90 |
| 7 |
Golembiewski, Melina E (13)
|
NIPACK |
|
6:16.76 |
- |
| 8 |
Guagliardo, Giovanna (11)
|
NIIRON |
|
6:36.85 |
- |
| 9 |
Kramer, Olivia S (12)
|
NIPACK |
7:29.12 |
7:53.50 |
24.38 |
|
Hill, Kayleigh A (15)
|
NIFAST |
5:58.36 |
NS |
- |