| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
LaRue, Carl V (14)
|
NIQUIK |
36.43 |
29.31 |
-7.12 |
| 2 |
Evans, Rebecca L (17)
|
NIFAST |
28.90 |
29.42 |
0.52 |
| 3 |
Wahl, Alec J (18)
|
NIWEBS |
30.08 |
31.03 |
0.95 |
| 4 |
Doescher, Mackenzi L (15)
|
NIFAST |
33.63 |
32.70 |
-0.93 |
| 5 |
Cupo, Michael A (14)
|
NIPACK |
38.71 |
33.57 |
-5.14 |
| 6 |
Miller, Piper P (15)
|
NIFAST |
43.94 |
34.24 |
-9.70 |
| 7 |
Romanick, Lauren A (15)
|
NIPACK |
33.63 |
35.33 |
1.70 |
| 8 |
Briggs, Katharine (14)
|
NIPACK |
40.69 |
35.61 |
-5.08 |
| 9 |
Bromage, Charlotte C (13)
|
NIQUIK |
37.77 |
36.00 |
-1.77 |
| 10 |
Hill, Kayleigh A (15)
|
NIFAST |
39.78 |
36.67 |
-3.11 |
| 11 |
Markham, Rory P (12)
|
NIFAST |
31.99 |
36.92 |
4.93 |
| 12 |
Podzorov, Maya L (11)
|
NIWEBS |
45.24 |
37.29 |
-7.95 |
| 13 |
Battista, Francesca F (15)
|
NIWEBS |
41.00 |
41.06 |
0.06 |
| 14 |
Crye, Jessie R (9)
|
NIFAST |
48.19 |
48.16 |
-0.03 |
| 15 |
Hinds, Benjamin W (9)
|
NIWEBS |
45.84 |
49.70 |
3.86 |
| 16 |
Visconte, Alexandria L (13)
|
NIFAST |
49.59 |
50.93 |
1.34 |
| 17 |
Gilbert, Natalie J (8)
|
NIQUIK |
57.53 |
51.61 |
-5.92 |
| 18 |
VanDamme, Avery M (11)
|
NIWEBS |
48.25 |
59.13 |
10.88 |
| 19 |
Cole, Bruno M (10)
|
NIPACK |
45.85 |
1:03.14 |
17.29 |
| 20 |
Visconte, Adriana E (11)
|
NIFAST |
1:09.34 |
1:04.58 |
-4.76 |
|
Obuhanych, Zachary J (11)
|
NIFAST |
37.82 |
NS |
- |
|
Oneil, Isabel D (12)
|
NIWEBS |
57.07 |
NS |
- |