| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Keyes, Celeste C (13)
|
NIUN-F |
5:32.66 |
5:28.62 |
-4.04 |
| 2 |
Golembiewski, Melina E (13)
|
NIPACK |
5:28.82 |
5:37.84 |
9.02 |
| 3 |
Geoca, Zoey L (14)
|
NISDSC |
6:00.17 |
5:52.12 |
-8.05 |
| 4 |
Giardina, Amanda G (13)
|
NISEAS |
5:44.77 |
5:53.83 |
9.06 |
| 5 |
Wallace, Emma E (13)
|
NIWEBS |
5:51.50 |
5:54.09 |
2.59 |
| 6 |
Tucker, Abby C (13)
|
NIFAST |
5:47.94 |
5:57.18 |
9.24 |
| 7 |
VanHouten, Ellie T (13)
|
NIPACK |
5:58.71 |
5:59.85 |
1.14 |
| 8 |
Kennedy, Madaliene F (14)
|
NISDSC |
6:08.30 |
6:00.12 |
-8.18 |
| 9 |
Diefendorf, Emma (13)
|
NIFAST |
6:33.41 |
6:17.84 |
-15.57 |
| 10 |
Polvino, Emilee E (14)
|
NIGRSC |
6:20.45 |
6:33.54 |
13.09 |
| 11 |
Daley, Regan D (13)
|
NIIRON |
6:30.14 |
6:38.80 |
8.66 |
| 12 |
Visconte, Alexandria L (13)
|
NIFAST |
6:43.62 |
6:50.31 |
6.69 |
|
Ingraham, Leah M (13)
|
NIGRSC |
5:35.71 |
NS |
- |