| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Reitz, Meghan E (18)
|
NIHAST |
5:27.21 |
5:27.97 |
0.76 |
| 2 |
Salonen-Roperti, Pearl M (15)
|
NIGCOM |
5:33.43 |
5:31.78 |
-1.65 |
| 3 |
Moore, Madison M (15)
|
NIVICT |
5:28.77 |
5:33.14 |
4.37 |
| 4 |
Federico, Sydney A (16)
|
NIGRSC |
5:37.22 |
5:37.92 |
0.70 |
| 5 |
Gartner, Sarah N (16)
|
NIWEBS |
5:28.34 |
5:44.39 |
16.05 |
| 6 |
Gallant, Hayley C (16)
|
NIPACK |
5:36.89 |
5:52.44 |
15.55 |
| 7 |
Stepanek, Livia G (15)
|
NIHAST |
6:04.86 |
6:05.93 |
1.07 |
| 8 |
Olson, Elaina A (15)
|
NIWEBS |
6:02.43 |
6:07.26 |
4.83 |
| 9 |
Geoca, Zoey L (15)
|
NISDSC |
5:48.66 |
6:08.95 |
20.29 |
| 10 |
Bonfiglio, Sara F (16)
|
NIGRSC |
5:58.08 |
6:11.12 |
13.04 |
| 11 |
Pratt, Samantha M (17)
|
NIGCOM |
5:46.80 |
6:13.10 |
26.30 |
| 12 |
Kennedy, Madaliene F (15)
|
NISDSC |
6:00.12 |
6:15.99 |
15.87 |
| 13 |
Polvino, Emilee E (15)
|
NIGRSC |
6:08.32 |
6:25.74 |
17.42 |