| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Aguilar, Nicole E (15)
|
FGWOW |
29.20 |
29.20 |
0.00 |
| 2 |
Cabonce, Michelle (17)
|
FGWOW |
32.05 |
29.65 |
-2.40 |
| 3 |
Giresi, Isbella M (16)
|
FGWOW |
31.72 |
31.21 |
-0.51 |
| 4 |
Mutschler, Faith (15)
|
FGWOW |
29.10 |
31.29 |
2.19 |
| 5 |
Weeter, Morgan O (15)
|
FGMART |
30.39 |
31.50 |
1.11 |
| 6 |
Patton, Robyn S (17)
|
FGBEST |
|
31.66 |
- |
| 7 |
Ruppert, Sophia G (16)
|
UNAT |
31.00 |
32.24 |
1.24 |
| 8 |
Gonzales-Mugaburu, Megan (15)
|
FGWOW |
34.59 |
32.97 |
-1.62 |
| 9 |
England, Jordan S (15)
|
FGWOW |
34.46 |
35.35 |
0.89 |
| 10 |
Kolesar, Madison R (16)
|
FGWOW |
|
35.38 |
- |
| 11 |
Perleche, Gabriela D (15)
|
UNAT |
38.46 |
40.01 |
1.55 |
|
Vensel, Ashley J (15)
|
FLT2 |
30.59 |
NS |
- |