| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Landow, Kayci L (8)
|
NIFKVL |
39.44 |
43.43 |
3.99 |
| 2 |
Owens, Annabelle R (8)
|
NIFKVL |
46.81 |
51.88 |
5.07 |
| 3 |
Jacobs, Acelynn K (6)
|
NISAAC |
55.46 |
52.69 |
-2.77 |
| 4 |
Graham, Gracyn M (7)
|
NIGVST |
58.63 |
56.57 |
-2.06 |
| 5 |
Stanbro, Allison L (8)
|
NIFKVL |
|
1:05.08 |
- |
| 6 |
Ellison, Paige A (8)
|
NIEMAC |
59.61 |
1:06.72 |
7.11 |
| 7 |
Hunt, Camdyn I (8)
|
NIGVST |
1:03.63 |
1:07.52 |
3.89 |
| 8 |
Herman, Genevieve G (7)
|
NIFKVL |
1:17.54 |
1:19.74 |
2.20 |
|
Tronetti, Seraphina G (7)
|
NIGVST |
|
DQ |
- |
|
Brown, Ellouise S (7)
|
NISAAC |
|
NS |
- |
|
John, Lily E (8)
|
NISAAC |
57.81 |
NS |
- |