| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kyre, Emily T (12)
|
OHKEY |
2:20.97 |
2:26.04 |
5.07 |
| 2 |
Hayes, Catherine L (12)
|
OHKEY |
2:53.35 |
2:37.01 |
-16.34 |
| 3 |
Herwig, Annabel G (12)
|
OHKEY |
2:46.70 |
2:41.39 |
-5.31 |
| 4 |
Spetz, Corinne E (12)
|
OHLAKY |
2:56.26 |
2:41.43 |
-14.83 |
| 5 |
Peck, Jordan E (12)
|
OHGMVY |
2:37.57 |
2:41.92 |
4.35 |
| 6 |
Burke, Kathleen (12)
|
OHGMVY |
|
2:43.40 |
- |
| 7 |
Kutter, Abigail T (12)
|
OHKEY |
2:52.09 |
2:46.93 |
-5.16 |
| 8 |
Lambert, Laina R (11)
|
OHSPY |
3:10.17 |
2:54.77 |
-15.40 |
| 9 |
Squibb, Jocelyn A (11)
|
OHLAKY |
|
2:55.44 |
- |
| 10 |
Kyre, Katie (11)
|
OHKEY |
3:02.37 |
2:57.51 |
-4.86 |
| 11 |
Wentzel, Charlotte M (11)
|
OHKEY |
3:12.60 |
3:02.55 |
-10.05 |
| 12 |
Simpson, Addison J (11)
|
OHLAKY |
|
3:03.58 |
- |
| 13 |
Schneider, Annabelle M (11)
|
OHLAKY |
|
3:09.45 |
- |
| 14 |
Woessner, Adelyn K (11)
|
OHKEY |
|
3:11.22 |
- |
| 15 |
Brinegar, Alicia M (12)
|
OHCFY |
|
3:14.70 |
- |
| 16 |
Miller, Lydia E (11)
|
OHLAKY |
3:28.83 |
3:15.60 |
-13.23 |
| 17 |
Krolikowski, Olivia A (11)
|
OHKEY |
|
3:27.89 |
- |
|
Petertonjes, Rachel A (11)
|
OHLAKY |
|
DQ |
- |