| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Woods, Samantha L (10)
|
OHLAKY |
2:37.98 |
2:30.44 |
-7.54 |
| 2 |
Fink, Sophia M (10)
|
OHLAKY |
2:54.83 |
2:31.18 |
-23.65 |
| 3 |
Lee, Madison R (10)
|
OHSPY |
2:38.71 |
2:35.67 |
-3.04 |
| 4 |
Lucas, Riley M (10)
|
OHCFY |
2:44.23 |
2:44.60 |
0.37 |
| 5 |
Kuntz, Brooklyne D (10)
|
OHSPY |
2:49.04 |
2:48.77 |
-0.27 |
| 6 |
Luebbe, Ashley M (10)
|
OHLAKY |
3:06.12 |
2:53.22 |
-12.90 |
| 7 |
Molina, Julia M (9)
|
OHLAKY |
3:02.08 |
3:01.56 |
-0.52 |
| 8 |
Kogler, Jordan D (9)
|
OHKEY |
3:24.26 |
3:01.81 |
-22.45 |
| 9 |
Fuller, Bailey M (10)
|
OHLAKY |
3:18.98 |
3:06.00 |
-12.98 |
| 10 |
Varvel, Olive A (10)
|
OHKEY |
3:21.20 |
3:17.52 |
-3.68 |
| 11 |
Dieter, Renee Y (10)
|
OHKEY |
|
3:22.24 |
- |
|
Pierstorff, Hannah M (10)
|
OHUCFY |
|
NS |
- |