| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sargent, Liam Q (14)
|
MAUMLY |
52.48 |
53.54 |
1.06 |
| 2 |
Ray, Prathit (14)
|
MAUMLY |
52.03 |
54.24 |
2.21 |
| 3 |
Manion, James P (14)
|
MAUMLY |
53.45 |
54.87 |
1.42 |
| 4 |
Moul, Thomas P (14)
|
MAUMLY |
53.78 |
54.92 |
1.14 |
| 5 |
Sahaida, Jack T (14)
|
MARY |
55.36 |
55.45 |
0.09 |
| 6 |
Turner, Seth M (13)
|
MAUMLY |
55.60 |
56.19 |
0.59 |
| 7 |
Whitmore, Korrigan E (14)
|
MABYNS |
56.51 |
56.92 |
0.41 |
| 8 |
Hopkins, Christian S (14)
|
MAUMLY |
58.75 |
58.39 |
-0.36 |
| 9 |
Drover, Adam R (14)
|
MAPAY |
1:00.90 |
1:00.42 |
-0.48 |
| 10 |
Emrick, Sebastian N (13)
|
MABYNS |
1:02.05 |
1:01.38 |
-0.67 |
| 11 |
Martin, Luke L (14)
|
MARY |
1:05.61 |
1:06.36 |
0.75 |
| 12 |
Strohl, Aidan c (13)
|
MABYNS |
1:15.57 |
1:13.49 |
-2.08 |