| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Padilla, Kenzie (13)
|
MAPAY |
58.77 |
58.91 |
0.14 |
| 2 |
Ramsey, Lauren M (13)
|
MARY |
1:01.52 |
1:03.32 |
1.80 |
| 3 |
Schermerhorn, Maren M (14)
|
MABYNS |
1:03.44 |
1:03.35 |
-0.09 |
| 4 |
Littler, Alicia (14)
|
MAWCAY |
1:01.16 |
1:03.36 |
2.20 |
| 5 |
Kane, Isabella (14)
|
MAWCAY |
1:04.09 |
1:04.69 |
0.60 |
| 6 |
Cunningham, Madison (14)
|
MARY |
1:05.07 |
1:06.39 |
1.32 |
| 7 |
Galef, Emma C (14)
|
MAUMLY |
1:04.82 |
1:07.83 |
3.01 |
| 8 |
Simpson, Katherine A (14)
|
MAWCAY |
1:06.58 |
1:08.13 |
1.55 |
| 9 |
Wiley, Keira E (14)
|
MAUMLY |
1:04.28 |
1:08.93 |
4.65 |
| 10 |
Kaczmarksi, Rachel (14)
|
MAWCAY |
1:13.47 |
1:11.64 |
-1.83 |
| 11 |
Nsereko, Sara (13)
|
MAWCAY |
1:14.75 |
1:13.38 |
-1.37 |
| 12 |
Skii, Lauren A (14)
|
MAWCAY |
|
1:18.69 |
- |
| 13 |
Shui, Lilly Y (14)
|
MAPAY |
1:18.42 |
1:23.72 |
5.30 |
|
Romberger, Brynn E (14)
|
MAPAY |
1:03.55 |
NS |
- |