| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Alessio, Madison A (12)
|
AMEFSC |
2:55.49 |
2:42.13 |
-13.36 |
| 2 |
Starostina, Milana M (11)
|
AMRXA |
3:30.99 |
2:42.32 |
-48.67 |
| 3 |
Dudkowski, Abigail E (12)
|
AMPRA |
2:51.82 |
2:42.39 |
-9.43 |
| 4 |
Melocchi, Grace R (12)
|
AMSASC |
3:04.80 |
2:42.88 |
-21.92 |
| 5 |
Siegel, Thea E (11)
|
AMHD |
2:44.98 |
2:43.29 |
-1.69 |
| 6 |
Langlois, Mackenzie M (12)
|
AMPTSC |
2:49.19 |
2:45.01 |
-4.18 |
| 7 |
Sheets, Elizabeth A (12)
|
AMHD |
2:51.33 |
2:47.19 |
-4.14 |
| 8 |
Sullivan, Riley A (12)
|
AMNAC |
2:53.17 |
2:51.05 |
-2.12 |
| 9 |
Shirley, Caroline B (11)
|
AMFAST |
3:04.69 |
2:58.02 |
-6.67 |
| 10 |
Weber, Laurel A (11)
|
AMHD |
3:20.98 |
3:18.06 |
-2.92 |
| 11 |
Vitale, Sarah B (12)
|
AMSASC |
3:35.87 |
3:23.16 |
-12.71 |
| 12 |
Grudowski, Natalie N (11)
|
AMMMSA |
3:40.99 |
3:30.84 |
-10.15 |
|
Gregorowicz, Abigail S (12)
|
AMPRA |
3:01.67 |
NS |
- |