| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Arnold, Lauren R (13)
|
MRBAD |
26.49 |
26.70 |
0.21 |
| 2 |
McDonough, Isabella T (14)
|
MRBAD |
|
27.57 |
- |
| 3 |
Han, Samantha S (13)
|
MRPAC |
27.60 |
27.86 |
0.26 |
| 4 |
Wentland, Emma A (14)
|
MRPAC |
27.61 |
27.90 |
0.29 |
| 5 |
McGrath, Caroline L (13)
|
MRBAD |
28.20 |
28.32 |
0.12 |
| 6 |
Marshall, Eileen C (14)
|
MRBAD |
29.11 |
28.74 |
-0.37 |
| 7 |
Kamal, Sarah M (14)
|
MRBGNW |
28.95 |
28.82 |
-0.13 |
| 8 |
Forrest, Jasmine (14)
|
MRSWAG |
29.18 |
28.88 |
-0.30 |
| 9 |
Kelly, Alanna M (13)
|
MREAST |
28.49 |
28.94 |
0.45 |
| 10 |
Nunez, Gabriela B (14)
|
MRRFAC |
28.74 |
28.97 |
0.23 |
| 11 |
Victory, Kathleen R (13)
|
MRBAD |
28.62 |
28.98 |
0.36 |
| 12 |
Kazibwe, Mary N (14)
|
MRBGNW |
28.03 |
29.01 |
0.98 |
| 13 |
Valentini, Ilaria (13)
|
MRPAC |
29.37 |
29.30 |
-0.07 |
| 14 |
Negron, Lorielle (14)
|
MRSWAG |
29.61 |
29.41 |
-0.20 |
| 15 |
Cook, April M (14)
|
MRRFAC |
29.42 |
29.51 |
0.09 |
| 15 |
Callaghan, Anna (13)
|
MRCFJV |
29.12 |
29.51 |
0.39 |
| 15 |
Genco, Olivia A (14)
|
MRPAC |
30.50 |
29.51 |
-0.99 |
| 18 |
Kelly, Colleen (13)
|
MRCFJV |
29.09 |
29.72 |
0.63 |
| 19 |
Crystal, Ella J (14)
|
MRBAD |
30.29 |
29.85 |
-0.44 |
| 20 |
Coquillette, Jackie A (14)
|
MRBAD |
29.69 |
29.90 |
0.21 |
| 21 |
Rabinowitz, Alana (14)
|
MRCFJV |
|
30.14 |
- |
| 22 |
Lew-Kiedrowska, Helena (13)
|
MREAST |
30.93 |
30.22 |
-0.71 |
| 23 |
Sheridan, Ally (14)
|
MRBAD |
31.87 |
31.11 |
-0.76 |
| 24 |
Ryan, Brooke (13)
|
MRCFJV |
31.28 |
31.15 |
-0.13 |
| 25 |
Galpotthawela, Mythri L (14)
|
MRBGNW |
30.91 |
31.16 |
0.25 |
| 26 |
Henriksson, Emma (13)
|
MRBAD |
30.69 |
31.18 |
0.49 |
| 27 |
Greenstein, Jessie B (13)
|
MRBGNW |
30.85 |
31.27 |
0.42 |
| 28 |
Chambers, Olivia G (13)
|
MREAST |
31.06 |
31.96 |
0.90 |
| 29 |
Lastras-Castrejon, Daniela (13)
|
MRCFJV |
33.19 |
32.22 |
-0.97 |
| 30 |
Argueta, Kirsten F (13)
|
MRPAC |
38.01 |
35.23 |
-2.78 |
| 31 |
Hatch, Evelyn A (13)
|
MRRFAC |
38.84 |
38.99 |
0.15 |