| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Liu, Jared (10)
|
MRNYSA |
43.40 |
42.37 |
-1.03 |
| 1 |
Dwyer, Aidan P (10)
|
MRCOND |
43.22 |
43.16 |
-0.06 |
| 2 |
Ha, Connor (10)
|
MRLIFE |
45.11 |
43.58 |
-1.53 |
| 2 |
Andros, Joseph B (10)
|
MRNDAC |
48.11 |
49.14 |
1.03 |
| 3 |
Kim, Jay (10)
|
MRCOND |
43.12 |
43.63 |
0.51 |
| 4 |
De Pierro, Jake C (10)
|
MRCOND |
44.55 |
43.79 |
-0.76 |
| 5 |
Rocchio, Carver R (10)
|
MRYBAR |
45.77 |
43.90 |
-1.87 |
| 6 |
Janec, Brady T (10)
|
MRSMC |
48.26 |
43.91 |
-4.35 |
| 7 |
Holland, Connor M (10)
|
MRNYSA |
44.63 |
44.33 |
-0.30 |
| 8 |
Sequeiros, Sebastian (10)
|
MRNYSA |
44.89 |
44.57 |
-0.32 |
| 9 |
Insdorf, Joshua C (10)
|
MRMWDA |
43.21 |
45.05 |
1.84 |
| 10 |
Puskas, Marcel (10)
|
MRNYSA |
45.36 |
45.59 |
0.23 |
| 11 |
Allen-Poole, Sean J (10)
|
MRAGMV |
46.81 |
45.75 |
-1.06 |
| 12 |
Song, Aidan S (10)
|
MRPAC |
45.07 |
46.02 |
0.95 |
| 13 |
Balcetis, Jonas (10)
|
MRBGNW |
44.68 |
46.12 |
1.44 |
| 14 |
Gauthier, Michael C (10)
|
MRCOND |
46.74 |
46.62 |
-0.12 |
| 15 |
Gibson, William M (10)
|
MRAGMV |
48.25 |
47.02 |
-1.23 |
| 16 |
Trenczer, Zachary D (10)
|
MRCOND |
47.65 |
47.12 |
-0.53 |
| 17 |
Variano, Matthew B (10)
|
MRRA |
46.64 |
47.21 |
0.57 |
| 18 |
Karamehmedovic, Jake (10)
|
MRLIFE |
46.54 |
47.33 |
0.79 |