| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pickrell, Nereide (10)
|
FGECAC |
33.50 |
32.01 |
-1.49 |
| 2 |
Glenn, Jensen L (10)
|
FGWOW |
36.70 |
34.57 |
-2.13 |
| 3 |
Edwards, Reef P (9)
|
FGFAST |
34.85 |
34.93 |
0.08 |
| 4 |
Meyer, Lauren E (9)
|
FGBRST |
33.13 |
35.25 |
2.12 |
| 4 |
Polston, Olivia E (10)
|
FGWOW |
36.19 |
35.25 |
-0.94 |
| 6 |
Potts, Meredith G (10)
|
FGBRST |
36.75 |
37.16 |
0.41 |
| 7 |
Saxena, Shivangi (10)
|
FGJDST |
40.46 |
37.65 |
-2.81 |
| 8 |
Gultepe, Neylin E (9)
|
FGWOW |
38.78 |
38.08 |
-0.70 |
| 9 |
Barone, Kendall (10)
|
FGLLL |
51.59 |
40.95 |
-10.64 |
| 10 |
Velez, Camryn F (10)
|
FGLLL |
44.28 |
42.27 |
-2.01 |
| 11 |
Vanegas, Stephania (10)
|
FGLLL |
|
42.43 |
- |
| 12 |
Brown, Daniela Z (9)
|
FGLLL |
49.14 |
46.57 |
-2.57 |
| 13 |
Cihowiak, Ava E (9)
|
FGJDST |
44.07 |
46.81 |
2.74 |
| 14 |
Spencer, Mary M (9)
|
FGFLA |
|
46.87 |
- |
| 15 |
Sadler, Isabelle M (9)
|
FGLLL |
|
48.36 |
- |
| 16 |
Gonzalez, Yadira (9)
|
FGBMSC |
53.56 |
49.34 |
-4.22 |
| 17 |
Delos Reyes, Conchessa S (9)
|
FGJDST |
51.19 |
49.61 |
-1.58 |
| 18 |
Heilner, Eleanora K (10)
|
FGJDST |
50.14 |
49.71 |
-0.43 |
| 19 |
Branstetter, Madyson A (10)
|
FGLLL |
|
57.24 |
- |
|
Campuzano, Gabriela M (9)
|
FGLLL |
35.40 |
NS |
- |
|
Velasquez, Camila (9)
|
FGWOW |
1:06.39 |
NS |
- |