| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Petranchuk, Sydney P (12)
|
FGJDST |
34.69 |
34.71 |
0.02 |
| 2 |
Adams, Ella (12)
|
FGJDST |
35.45 |
35.23 |
-0.22 |
| 3 |
Battles, Alyssa K (12)
|
FGJDST |
33.74 |
35.37 |
1.63 |
| 4 |
Rosa, Anna G (12)
|
FGYSPB |
33.36 |
35.78 |
2.42 |
| 5 |
Devendorf, Briallen R (11)
|
FGYSPB |
35.29 |
37.39 |
2.10 |
| 6 |
Rubin, Marley A (11)
|
FGYSPB |
37.16 |
38.08 |
0.92 |
| 7 |
Roy, Lillian K (11)
|
FGLLL |
42.35 |
40.46 |
-1.89 |
| 8 |
Partin, Riley F (11)
|
FGMART |
40.56 |
41.67 |
1.11 |
| 9 |
Pointer, Madison A (11)
|
FGJDST |
|
43.92 |
- |
| 10 |
Blair, Jenna A (11)
|
FGMART |
48.40 |
48.18 |
-0.22 |
| 11 |
Guisao, Kimey (11)
|
FGBMSC |
47.44 |
50.66 |
3.22 |
|
Carmona, Valeria (11)
|
FGYSPB |
53.59 |
DQ |
- |
|
Rasmussen, Isabella M (12)
|
FGLLL |
|
NS |
- |
|
Salcedo, Sara F (11)
|
FGLLL |
37.99 |
NS |
- |