| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Velte, Lucy L (12)
|
MVMV |
31.21 |
31.18 |
-0.03 |
| 2 |
Miller, Karina I (12)
|
ILIL |
32.14 |
32.13 |
-0.01 |
| 3 |
Kret, Tamara E (12)
|
ILIL |
32.41 |
32.44 |
0.03 |
| 4 |
Glinko, Claudia (12)
|
ILIL |
32.11 |
32.49 |
0.38 |
| 5 |
Roggi, Lia M (11)
|
ILIL |
32.98 |
32.96 |
-0.02 |
| 6 |
Olander, Brenna M (12)
|
OKOK |
34.46 |
33.51 |
-0.95 |
| 7 |
Simmons, Bobbi E (11)
|
MNMSI |
34.30 |
33.54 |
-0.76 |
| 8 |
Bike, Thea L (11)
|
ILIL |
34.28 |
33.73 |
-0.55 |
| 9 |
Mingmuang, Briana E (11)
|
ILIL |
33.54 |
33.82 |
0.28 |
| 10 |
Jian-gross, Jiayin (12)
|
MNMSI |
34.36 |
33.96 |
-0.40 |
| 11 |
Wilkey, Claire M (11)
|
MNMSI |
34.16 |
34.32 |
0.16 |
| 12 |
McAfee, Sofia M (12)
|
ARAR |
|
34.50 |
- |
| 13 |
Koenig-Song, Adeline (11)
|
ILIL |
33.96 |
34.60 |
0.64 |
| 14 |
Hinrich, Kenzie E (12)
|
SDSD |
33.54 |
34.74 |
1.20 |
| 15 |
Henderson, Georgia K (12)
|
MWMW |
29.80 |
34.77 |
4.97 |
| 16 |
Elzey, Aika C (12)
|
ILIL |
34.22 |
35.57 |
1.35 |
| 17 |
Aviles, Izabella C (11)
|
ILIL |
33.93 |
35.65 |
1.72 |
| 18 |
Shryock, Madison G (11)
|
MVMV |
|
35.70 |
- |
| 19 |
Jernigan, Jasper H (12)
|
OKOK |
|
35.80 |
- |
| 20 |
Gemar, Berkley M (12)
|
MWMW |
|
35.82 |
- |
| 21 |
Percifull, Abbigail H (11)
|
ARAR |
|
36.03 |
- |
| 22 |
McDonagh, Ella J (12)
|
NDND |
|
36.94 |
- |
| 23 |
Wettlaufer, Jocelyn A (12)
|
NDND |
|
37.48 |
- |
| 24 |
Sparks, Sydney R (12)
|
SDSD |
|
37.83 |
- |