| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McKillop, Jospeh E (10)
|
ILFMC |
43.48 |
41.35 |
-2.13 |
| 2 |
Williams, Joel K (10)
|
ILSST |
47.57 |
41.52 |
-6.05 |
| 3 |
Torpey, Blake W (10)
|
ILFOX |
44.75 |
41.76 |
-2.99 |
| 4 |
Rybaski, Hayden C (10)
|
ILSCST |
43.50 |
42.31 |
-1.19 |
| 5 |
Ko, Jordan J (10)
|
ILGSC |
44.51 |
42.34 |
-2.17 |
| 6 |
Sagun, Paxton V (10)
|
ILWILD |
45.83 |
43.84 |
-1.99 |
| 7 |
Bulger, Christian G (10)
|
ILRMSC |
47.58 |
44.50 |
-3.08 |
| 8 |
Papanicholas, Luke J (10)
|
ILGSC |
46.54 |
44.74 |
-1.80 |
| 9 |
Czosnyka, David (10)
|
ILPAC |
48.40 |
44.79 |
-3.61 |
| 10 |
Rimas, Edward A (10)
|
ILNEPT |
44.70 |
44.91 |
0.21 |
| 11 |
Postovalov, Lucas A (10)
|
ILBRRY |
46.81 |
45.58 |
-1.23 |
| 12 |
Karasek, Nicholas (10)
|
ILPPD |
46.87 |
45.74 |
-1.13 |
| 13 |
Millhollin, Easton A (9)
|
ILDDST |
47.18 |
46.10 |
-1.08 |
| 14 |
Kovalkov, Maksim (10)
|
ILBRRY |
47.24 |
46.25 |
-0.99 |
| 15 |
Xu, Evan J (10)
|
ILFOX |
46.29 |
46.80 |
0.51 |
| 16 |
Landorf, George A (10)
|
ILLIFE |
46.98 |
46.85 |
-0.13 |
| 17 |
Peluso, Paul M (9)
|
ILFMC |
47.95 |
46.89 |
-1.06 |
| 18 |
Pavlovic, Milan (10)
|
ILHOSC |
46.90 |
47.44 |
0.54 |
| 19 |
Jevne, Stephen G (10)
|
ILBRRY |
47.94 |
48.06 |
0.12 |
| 20 |
Pugachev, Daniel A (10)
|
ILPAC |
40.41 |
48.58 |
8.17 |
| 21 |
Becker, Samuel J (10)
|
ILLIFE |
46.78 |
48.81 |
2.03 |
| 22 |
Casey, Jack L (10)
|
ILJPD |
40.41 |
49.24 |
8.83 |
| 23 |
Olivo, Abel (10)
|
ILTIDE |
50.56 |
49.81 |
-0.75 |
| 24 |
Banicz, Alexander Z (10)
|
ILACAD |
49.67 |
50.22 |
0.55 |
| 25 |
Diforti, Dominic T (10)
|
ILNAC |
50.55 |
50.26 |
-0.29 |
| 26 |
Banini, Cristian K (10)
|
ILSPD |
49.48 |
51.17 |
1.69 |
| 27 |
Diviacchi, Giovanni R (9)
|
ILMMSC |
51.61 |
54.10 |
2.49 |